Chlorine dioxide is a broad-spectrum biocide with proven efficacy against a range of microbial organisms such as bacteria, viruses, protozoa, yeasts, mycobacteria and bacterial spores. Originally used in water treatment and food industries, chlorine dioxide has now been adopted as the go-to high-level disinfectant in medical, pharmaceutical, veterinary and laboratory settings.
The mechanism through which chlorine dioxide achieves its potent biocidal effect is oxidation1. Oxidation involves the oxidising agent sequestering electrons from the microorganism, this causes a molecular imbalance which inevitably leads to the microorganism’s death. The main advantage of using oxidising biocides over non oxidising biocides (alcohols, quaternary ammonium compounds), is the inability for microorganisms to develop resistance2, as well as the broad range of efficacy1.
The comparison of chlorine dioxide with other oxidisers, such as hydrogen peroxide is of interest. Whilst hydrogen peroxide and other oxidisers work through the same mechanisms as chlorine dioxide, they have been shown to be less effective against microbes3. This is attributed to chlorine dioxide’s unique ability to penetrate the microorganism’s 3D protein structure3, and thus better oxidise the molecules. Additionally, chlorine dioxide forms no hazardous by-products4, and is less harmful to people and equipment than other common biocides such as hydrogen peroxide and chlorine2.
Chlorine dioxide is supported through the Biocidal Product Regulation for use in product types 2, 3, 4, 5, 11 and 12 and is also approved the United States Environmental Protection Agency.
- Block, S., Knapp, J. and Battisti, D. (2001). Disinfection, sterilization, and preservation. Philadelphia: Lippincott Williams & Wilkins, pp.215-227.
- Noszticzius, Z., Wittmann, M., Kály-Kullai, K., Beregvári, Z., Kiss, I., Rosivall, L. and Szegedi, J. (2013). Chlorine Dioxide Is a Size-Selective Antimicrobial Agent. PLoS ONE, 8(11), p.e79157.
- Finnegan, M., Linley, E., Denyer, S.P., McDonnell, G., Simons, C., Maillard, J.Y. (2010) Mode of action of hydrogen peroxide and other oxidizing agents: differences between liquid and gas forms, Journal of Antimicrobial Chemotherapy, 65(10), pp. 2108–2115.
- Al-Otoum, F., Al-Ghouti, M., Ahmed, T., Abu-Dieyeh, M. and Ali, M. (2016). Disinfection by-products of chlorine dioxide (chlorite, chlorate, and trihalomethanes): Occurrence in drinking water in Qatar. Chemosphere, 164, pp.649-656.